Cloning and expression of human granulocyte-macrophage colony stimulating factor (hGM-CSF) in Pichia pastoris
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چکیده
منابع مشابه
Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris
Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...
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ژورنال
عنوان ژورنال: Science and Technology Development Journal
سال: 2013
ISSN: 1859-0128,1859-0128
DOI: 10.32508/stdj.v16i3.1635